第36卷第6期
山东中医药大学学报
JOURNAL OF SHANDONG UNIVERSITY OF TCM 山东中医药大学学报
Vol.36,No.6.2012第36Nov 卷第6期
年月20121111月2012年
·中药研究·
GC -MS 法分析酸枣仁汤及其各单味药的挥发性成分
孙志翠1,刘西建1,郭
炜3,裴强伟1,吕青涛2,韩
涛1,王
欣1
(1.山东中医药大学基础医学院;2.山东中医药大学药学院;3.山东中医药大学实验中心,山东济南250355)
[摘要]目的:比较酸枣仁汤及各单味药水蒸气蒸馏物化学成分的差异,从而鉴定出该方经水煎煮后产生的新成分。方法:采用水蒸气蒸馏法制备供试品溶液,运用气相色谱/质谱联用(GC -MS )对复方及各单味药的水蒸气蒸馏物进行成分分析。结果:全方共鉴定出80种化合物,其中共有峰酸枣仁占17种,茯苓4种,知母17种,甘草1种,川芎
37种,还发现20种不属于任何一味中药,考虑可能为酸枣仁汤经水煎煮后产生的新成分,且以1-苯基-1-戊酮的含
量最高,(S )-1-甲基-4-(5-甲基-1-甲基-4-己烯基)-环己烯次之。结论:酸枣仁汤单煎与合煎水蒸气蒸馏物的化学成分存在显著差异,研究结果为酸枣仁汤及各单味药的GC -MS 研究提供了方法学基础,也为探讨酸枣仁汤镇静催眠药效物质基础提供了一定依据。
[关键词]酸枣仁汤;GC -MS ;水蒸气蒸馏;挥发性成分[中图分类号]R286.0
[文献标识码]A
[文章编号]1007-659X (2012)06-0534-03
Analysis of volatile composition of Suanzaoren decoction and its individual herb by GC -MS SUN Zhi -cui 1,LIU Xi -jian 1,GUO Wei 3,PEI Qiang -wei 1,LV Qing -tao 2,HAN Tao 1,WANG xin 1(1.BasicMedical College ,Shandong University of Traditional Chinese Medicine ,Jinan 250355,China ;2.Collegeof pharmacy ,Shan dong University of Traditional Chinese Medicine ,Jinan 250355,China ;3.ExperimentalCenter of Shandong University of Traditional Chinese Medicine ,Jinan 250355,China )
[Abstract ]Objective :To compare the separated chemical components from volatile composition of Suanzaoren
decoction (SZRD )with its individual herb ,to identify new components from the volatiles composition of SZRD.
Methods :The volatile compositions of SZRD were obtained using a method of steam distillation.The individual chemical components were further characterized using gas chromatography -mass spectrometry (GC -MS ).Results :In total ,80compounds were identified ,17were belong to semen ziziphi spinosae ,4were poria ,17were rhizome anemarrhena ,1was radix glycyrrhizae and 37were rhizoma chuanxiong.However ,20compounds were not able to be identified as any of Chinese traditional medicine.Thus ,they were suggested as new components from SZRD in which the most of them are 1-phenyl -1-pentanone and the most rest were (S )-1-methyl -4-(5-methyl -1-methylene -4-hexenyl )-cyclohexene.Conclusion :There is a substantial difference of the chemical composition between SZRD and its individual herb.The study proposes an approach to analysis SZRD and its individual herb by GC -MS and helps us to study the therapeutic role of SZRD as sedative hypnotics.
[Key words ]Suanzaoren decoction ;GC -MS ;steam distillation ;volatile composition
酸枣仁汤出自东汉·张仲景《金匮要略》,为滋养安神之代表方[1]。本方现代临床多用于治疗顽固性失眠及以情绪或神志障碍为主要表现的神经精神系统疾病[2]。王育虎等[3]曾观察酸枣仁汤对大鼠神经元、血-脑脊液屏障超微结构的影响,以探讨酸枣仁汤治疗失眠症的作用机制,证明该方可通过血-脑
[收稿日期]2012-04-09
[基金项目]山东省优秀中青年科学家科研奖励基金(编号:BS2011YY054)
[作者简介]孙志翠(1987-),女,天津人,2010年级硕士研究生,主要从事中药复方作用机制与药效物质基础研究。
[通讯作者]王欣,博士,副教授,主要从事方剂效用机制及物质基础研究,E -mail :xinw0422@126.com。
脊液屏障发挥作用。由于能进入血-脑脊液屏障的皆为相对分子质量<400~500的小分子亲脂性物质[4],故考虑方中的挥发性成分具有主要作用。本研究拟采用GC -MS 对酸枣仁汤及其各单味药的水蒸气蒸馏物进行成分分析,以探讨酸枣仁汤镇静催眠药效物质基础。1试药与仪器
酸枣仁、茯苓、川芎、知母、甘草药材均购于山东中医药大学中鲁医院,经鉴定为正品;乙酸乙酯(天津四友精细化学品有限公司);无水硫酸钠(分析纯)(天津市化学试剂厂)。Agilent5975B 气相色谱-质谱联用仪(上海天普色谱仪有限公司);GC -MS solution
534
第36卷第6期
山东中医药大学学报
JOURNAL OF SHANDONG UNIVERSITY OF TCM 山东中医药大学学报
Vol.36,No.6.2012第36Nov 卷第6期
年月20121111月2012年
·中药研究·
GC -MS 法分析酸枣仁汤及其各单味药的挥发性成分
孙志翠1,刘西建1,郭
炜3,裴强伟1,吕青涛2,韩
涛1,王
欣1
(1.山东中医药大学基础医学院;2.山东中医药大学药学院;3.山东中医药大学实验中心,山东济南250355)
[摘要]目的:比较酸枣仁汤及各单味药水蒸气蒸馏物化学成分的差异,从而鉴定出该方经水煎煮后产生的新成分。方法:采用水蒸气蒸馏法制备供试品溶液,运用气相色谱/质谱联用(GC -MS )对复方及各单味药的水蒸气蒸馏物进行成分分析。结果:全方共鉴定出80种化合物,其中共有峰酸枣仁占17种,茯苓4种,知母17种,甘草1种,川芎
37种,还发现20种不属于任何一味中药,考虑可能为酸枣仁汤经水煎煮后产生的新成分,且以1-苯基-1-戊酮的含
量最高,(S )-1-甲基-4-(5-甲基-1-甲基-4-己烯基)-环己烯次之。结论:酸枣仁汤单煎与合煎水蒸气蒸馏物的化学成分存在显著差异,研究结果为酸枣仁汤及各单味药的GC -MS 研究提供了方法学基础,也为探讨酸枣仁汤镇静催眠药效物质基础提供了一定依据。
[关键词]酸枣仁汤;GC -MS ;水蒸气蒸馏;挥发性成分[中图分类号]R286.0
[文献标识码]A
[文章编号]1007-659X (2012)06-0534-03
Analysis of volatile composition of Suanzaoren decoction and its individual herb by GC -MS SUN Zhi -cui 1,LIU Xi -jian 1,GUO Wei 3,PEI Qiang -wei 1,LV Qing -tao 2,HAN Tao 1,WANG xin 1(1.BasicMedical College ,Shandong University of Traditional Chinese Medicine ,Jinan 250355,China ;2.Collegeof pharmacy ,Shan dong University of Traditional Chinese Medicine ,Jinan 250355,China ;3.ExperimentalCenter of Shandong University of Traditional Chinese Medicine ,Jinan 250355,China )
[Abstract ]Objective :To compare the separated chemical components from volatile composition of Suanzaoren
decoction (SZRD )with its individual herb ,to identify new components from the volatiles composition of SZRD.
Methods :The volatile compositions of SZRD were obtained using a method of steam distillation.The individual chemical components were further characterized using gas chromatography -mass spectrometry (GC -MS ).Results :In total ,80compounds were identified ,17were belong to semen ziziphi spinosae ,4were poria ,17were rhizome anemarrhena ,1was radix glycyrrhizae and 37were rhizoma chuanxiong.However ,20compounds were not able to be identified as any of Chinese traditional medicine.Thus ,they were suggested as new components from SZRD in which the most of them are 1-phenyl -1-pentanone and the most rest were (S )-1-methyl -4-(5-methyl -1-methylene -4-hexenyl )-cyclohexene.Conclusion :There is a substantial difference of the chemical composition between SZRD and its individual herb.The study proposes an approach to analysis SZRD and its individual herb by GC -MS and helps us to study the therapeutic role of SZRD as sedative hypnotics.
[Key words ]Suanzaoren decoction ;GC -MS ;steam distillation ;volatile composition
酸枣仁汤出自东汉·张仲景《金匮要略》,为滋养安神之代表方[1]。本方现代临床多用于治疗顽固性失眠及以情绪或神志障碍为主要表现的神经精神系统疾病[2]。王育虎等[3]曾观察酸枣仁汤对大鼠神经元、血-脑脊液屏障超微结构的影响,以探讨酸枣仁汤治疗失眠症的作用机制,证明该方可通过血-脑
[收稿日期]2012-04-09
[基金项目]山东省优秀中青年科学家科研奖励基金(编号:BS2011YY054)
[作者简介]孙志翠(1987-),女,天津人,2010年级硕士研究生,主要从事中药复方作用机制与药效物质基础研究。
[通讯作者]王欣,博士,副教授,主要从事方剂效用机制及物质基础研究,E -mail :xinw0422@126.com。
脊液屏障发挥作用。由于能进入血-脑脊液屏障的皆为相对分子质量<400~500的小分子亲脂性物质[4],故考虑方中的挥发性成分具有主要作用。本研究拟采用GC -MS 对酸枣仁汤及其各单味药的水蒸气蒸馏物进行成分分析,以探讨酸枣仁汤镇静催眠药效物质基础。1试药与仪器
酸枣仁、茯苓、川芎、知母、甘草药材均购于山东中医药大学中鲁医院,经鉴定为正品;乙酸乙酯(天津四友精细化学品有限公司);无水硫酸钠(分析纯)(天津市化学试剂厂)。Agilent5975B 气相色谱-质谱联用仪(上海天普色谱仪有限公司);GC -MS solution
534