复方中药提取物抗白色念珠菌活性及其主要成分
摘要:研究由苦参､土茯苓和白鲜皮组成的复方中药提取物的抗白色念珠菌活性及主要成分｡采用肉汤微量稀释法, 测定复方中药提取物对白色念珠菌的抗菌活性, 并采用化学方法对中药复方提取液进行成分预试｡结果显示, 提取物具明显的抑制白色念珠菌生长的活性; 经理化鉴别试验显示, 该复方提取物主要含有多糖､黄酮､生物碱三大类成分｡表明多糖､黄酮和生物碱这三大类成分可能是复方提取物发挥抗白色念珠菌活性的重要成分, 但确定其发挥作用的具体成分还需进一步的研究｡
关键词:复方中药; 白色念珠菌; 苦参; 土茯苓; 白鲜皮
Study on Anti-Candida albicans Activity and Major Components of Compound Chinese Medicine Extracts
Abstract: The anti-Candida albicans activity and major components of compound Chinese Medicine extracts composed of Sophora flavescens, Smilax glabra and Cortex dictamni were researched. The broth microdilution antifungal susceptibility test was applied to study antibacterial activity of compound extracts to Candida albicans. The component pretesting of compound extracts was carried out by chemistry method. The results revealed that extracts had obvious anti-Candida albicans activity, and polysaccharides, flavonoids and alkaloids were the major components of compound extracts. It＇s possible that the three components were the significant components of exerting the antifungal activity. However, the identification of concrete elements still needed further study.
Key words: compound Chinese medicine; Candida albicans; Sophora flavescens; Smilax glabra; Cortex dictamni
白色念珠菌(Candida albicans) 是机会致病菌, 可致人和动物念珠菌病[1]｡近年来, 其感染率不断上升[2],且耐药菌株亦逐年增多｡而现有治疗药物存在易产生毒副反应､过敏反应等缺点｡中药材制剂因具有高效低毒､且不易产生耐药性等优点, 从中寻找新型抗白色念珠菌药物成为研究的新趋势[3]｡
苦参(Radix Sophorae Flavescentis,RSF)､土茯苓(Rhizome Smilacis Glabrae,RSG)及白鲜皮(Cortex Dictamni,CD) 为传统中药材｡近年的研究表明, 苦参对细菌及多种皮肤真菌有不同程度的抑制作用[4-7]｡土茯苓抑菌范围广, 活性强, 是重要的抗细菌药
物[8-9]｡而白鲜皮为中医皮肤科常用药物, 对部分皮肤真菌及白色念珠菌､金色葡萄球菌等均有一定的抑菌作用[10-12]｡本研究对这3种中药材的复方中药提取物进行了药敏试验, 观察其对白色念珠菌的抑菌效果, 并对提取物进行了成分预试
复方中药提取物抗白色念珠菌活性及其主要成分
摘要:研究由苦参､土茯苓和白鲜皮组成的复方中药提取物的抗白色念珠菌活性及主要成分｡采用肉汤微量稀释法, 测定复方中药提取物对白色念珠菌的抗菌活性, 并采用化学方法对中药复方提取液进行成分预试｡结果显示, 提取物具明显的抑制白色念珠菌生长的活性; 经理化鉴别试验显示, 该复方提取物主要含有多糖､黄酮､生物碱三大类成分｡表明多糖､黄酮和生物碱这三大类成分可能是复方提取物发挥抗白色念珠菌活性的重要成分, 但确定其发挥作用的具体成分还需进一步的研究｡
关键词:复方中药; 白色念珠菌; 苦参; 土茯苓; 白鲜皮
Study on Anti-Candida albicans Activity and Major Components of Compound Chinese Medicine Extracts
Abstract: The anti-Candida albicans activity and major components of compound Chinese Medicine extracts composed of Sophora flavescens, Smilax glabra and Cortex dictamni were researched. The broth microdilution antifungal susceptibility test was applied to study antibacterial activity of compound extracts to Candida albicans. The component pretesting of compound extracts was carried out by chemistry method. The results revealed that extracts had obvious anti-Candida albicans activity, and polysaccharides, flavonoids and alkaloids were the major components of compound extracts. It＇s possible that the three components were the significant components of exerting the antifungal activity. However, the identification of concrete elements still needed further study.
Key words: compound Chinese medicine; Candida albicans; Sophora flavescens; Smilax glabra; Cortex dictamni
白色念珠菌(Candida albicans) 是机会致病菌, 可致人和动物念珠菌病[1]｡近年来, 其感染率不断上升[2],且耐药菌株亦逐年增多｡而现有治疗药物存在易产生毒副反应､过敏反应等缺点｡中药材制剂因具有高效低毒､且不易产生耐药性等优点, 从中寻找新型抗白色念珠菌药物成为研究的新趋势[3]｡
苦参(Radix Sophorae Flavescentis,RSF)､土茯苓(Rhizome Smilacis Glabrae,RSG)及白鲜皮(Cortex Dictamni,CD) 为传统中药材｡近年的研究表明, 苦参对细菌及多种皮肤真菌有不同程度的抑制作用[4-7]｡土茯苓抑菌范围广, 活性强, 是重要的抗细菌药
物[8-9]｡而白鲜皮为中医皮肤科常用药物, 对部分皮肤真菌及白色念珠菌､金色葡萄球菌等均有一定的抑菌作用[10-12]｡本研究对这3种中药材的复方中药提取物进行了药敏试验, 观察其对白色念珠菌的抑菌效果, 并对提取物进行了成分预试