常用载体及相关通用引物
默认分类 2008-10-06 13:16:31 阅读512 评论0 字号：大中小
载体
pBlueScriptSK(+) pcDNA3 pcDNA3.1(+)
pcDNA3.0(+)/myc-His C/A/B pcDNA3.1(+)/myc-His C/A/B pcDNA3.1(+)/myc-His/LacZ pcDNA3.1/His C/A/B pcDNA3.1/His/LacZ pcDNA3.1/Hygro(-) pcDNA3.1/Hygro(+) pcDNA3.1/Hygro/LacZ pcDNA3.1/V5-His C/A/B pcDNA3.1/V5-His/LacZ pcDNA3.1/V5-His-TOPO pcDNA3.1/V5-His-TOPO/LacZ pcDNA3.1/Zeo(-) pcDNA3.1/Zeo(+) pcDNA3.1/Zeo/CAT pColdI DNA pColdII DNA pColdIII DNA pColdIV DNA pDream2.1 pEGFP-N1,2,3 pET15b pET20b
一端
M13F(-77)/M13F(-47)/M13F/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 p-GEX3’ p-GEX3’ p-GEX3’ p-GEX3’
H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5 T7 T7
另一端
M13R(-88)/M13R(-48)/M13R/T3 BGH
pCDNA3.1R/BGH BGH
pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH SP6
pEGFP-N-3’/pEGFP-C-5’/pEGFP-C-3' T7ter T7ter
pET22b T7 pET28a T7 pET28b T7 pET30b T7
pFastBac Dual pEGFP-C-3' pFastBac HT A,B,C pEGFP-C-3' pFastBac1 pEGFP-C-3'
pGEM-T Easy M13F(-77)/M13F(-47)/M13F/T7 pGEX-4T-1/3 p-GEX5’ pGS-21a
p-GEX5’
pLenti6/V5-D-TOPO M13F(-47)/M13F/T7 pLenti6/V5-GW/lacZ M13F(-47)/M13F/T7 pMAL-p2x M13F(-47)/M13F/PBV220R pMD18-T M13F(-77)/M13F(-47)/M13F pMD19-T
M13F(-77)/M13F(-47)/M13F pMTBiPV5-His GFP p-GEX3’/ M13F(-47)/M13F pPCR script M13F(-77)/M13F(-47)/M13F/T7 pPIC3.5 5’AOX pPIC9 5’AOX pPICZ C/A/B 5’AOX
pPICZalpha C/A/B 5’AOX/α-Factor
pSecTag2 A,B,C H1.3F/pCMV-F /pEGFP-N-5/T7 pTWIN T7
pUC57 M13F(-77)/M13F(-47)/M13F pUC18 M13F(-47)/M13F pUC19 M13F(-47)/M13F 其他 ……
注：1）排列顺序为：有左到右==（离插入为点）由远到近； 2）若有多个引物，红色的为首选引物：
3）选择测序通用引物时，以距离插入位点约50碱基为佳； 4）同时有T7，T7ter的载体，单反应首选T7ter；
T7ter T7ter T7ter T7ter
M13R(-88)/M13R(-48)/M13R p-GEX3’ T7ter
M13R(-48)/M13R/T3 M13R(-48)/M13R/T3 M13R(-48)
M13R(-88)/M13R(-48)/M13R M13R(-88)/M13R(-48)/M13R
M13R(-88)/M13R(-48)/M13R /pEGFP-C-3'/BGH M13R(-88)/M13R(-48)/M13R/T3 3’AOX 3’AOX 3’AOX 3’AOX BGH T7ter
M13R(-88)/M13R(-48)/M13R M13R(-48)/M13R M13R(-48)/M13R ……
5）同时有T7，pCDNA3.1R的载体，单反应首选pCDNA3.1R ； 6）同时有T7，BGH的载体，单反应首选BGH； 7）同时有pCMV-F，BGH的载体，单反应首选BGH；
常用载体及相关通用引物
默认分类 2008-10-06 13:16:31 阅读512 评论0 字号：大中小
载体
pBlueScriptSK(+) pcDNA3 pcDNA3.1(+)
pcDNA3.0(+)/myc-His C/A/B pcDNA3.1(+)/myc-His C/A/B pcDNA3.1(+)/myc-His/LacZ pcDNA3.1/His C/A/B pcDNA3.1/His/LacZ pcDNA3.1/Hygro(-) pcDNA3.1/Hygro(+) pcDNA3.1/Hygro/LacZ pcDNA3.1/V5-His C/A/B pcDNA3.1/V5-His/LacZ pcDNA3.1/V5-His-TOPO pcDNA3.1/V5-His-TOPO/LacZ pcDNA3.1/Zeo(-) pcDNA3.1/Zeo(+) pcDNA3.1/Zeo/CAT pColdI DNA pColdII DNA pColdIII DNA pColdIV DNA pDream2.1 pEGFP-N1,2,3 pET15b pET20b
一端
M13F(-77)/M13F(-47)/M13F/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5/T7 p-GEX3’ p-GEX3’ p-GEX3’ p-GEX3’
H1.3F/pCMV-F /pEGFP-N-5/T7 H1.3F/pCMV-F /pEGFP-N-5 T7 T7
另一端
M13R(-88)/M13R(-48)/M13R/T3 BGH
pCDNA3.1R/BGH BGH
pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH pCDNA3.1R/BGH SP6
pEGFP-N-3’/pEGFP-C-5’/pEGFP-C-3' T7ter T7ter
pET22b T7 pET28a T7 pET28b T7 pET30b T7
pFastBac Dual pEGFP-C-3' pFastBac HT A,B,C pEGFP-C-3' pFastBac1 pEGFP-C-3'
pGEM-T Easy M13F(-77)/M13F(-47)/M13F/T7 pGEX-4T-1/3 p-GEX5’ pGS-21a
p-GEX5’
pLenti6/V5-D-TOPO M13F(-47)/M13F/T7 pLenti6/V5-GW/lacZ M13F(-47)/M13F/T7 pMAL-p2x M13F(-47)/M13F/PBV220R pMD18-T M13F(-77)/M13F(-47)/M13F pMD19-T
M13F(-77)/M13F(-47)/M13F pMTBiPV5-His GFP p-GEX3’/ M13F(-47)/M13F pPCR script M13F(-77)/M13F(-47)/M13F/T7 pPIC3.5 5’AOX pPIC9 5’AOX pPICZ C/A/B 5’AOX
pPICZalpha C/A/B 5’AOX/α-Factor
pSecTag2 A,B,C H1.3F/pCMV-F /pEGFP-N-5/T7 pTWIN T7
pUC57 M13F(-77)/M13F(-47)/M13F pUC18 M13F(-47)/M13F pUC19 M13F(-47)/M13F 其他 ……
注：1）排列顺序为：有左到右==（离插入为点）由远到近； 2）若有多个引物，红色的为首选引物：
3）选择测序通用引物时，以距离插入位点约50碱基为佳； 4）同时有T7，T7ter的载体，单反应首选T7ter；
T7ter T7ter T7ter T7ter
M13R(-88)/M13R(-48)/M13R p-GEX3’ T7ter
M13R(-48)/M13R/T3 M13R(-48)/M13R/T3 M13R(-48)
M13R(-88)/M13R(-48)/M13R M13R(-88)/M13R(-48)/M13R
M13R(-88)/M13R(-48)/M13R /pEGFP-C-3'/BGH M13R(-88)/M13R(-48)/M13R/T3 3’AOX 3’AOX 3’AOX 3’AOX BGH T7ter
M13R(-88)/M13R(-48)/M13R M13R(-48)/M13R M13R(-48)/M13R ……
5）同时有T7，pCDNA3.1R的载体，单反应首选pCDNA3.1R ； 6）同时有T7，BGH的载体，单反应首选BGH； 7）同时有pCMV-F，BGH的载体，单反应首选BGH；